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KMID : 0371619950110020215
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Journal of Wonkwang Medical Science
1995 Volume.11 No. 2 p.215 ~ p.226
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Roles of TGF-β1 in Formation of Stricture after Urethral injury in Rats
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Seo Ill-Young
Rim Joung-Sik
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Abstract
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Transforming growth factor-β_1 (TGF-β_1)is a homodimeric peptide of 25kDa involved in many fundamental biological processes including cell growth, extracellular matrix deposition and degradation, and inflammatory responses. Especially TGF-β_1 is released by platelet and inflammatory cells, and it affects all phases of the wound healing after injury. It contributes to the regulation of fibroblast chemotaxis and proliferation, and also controls the synthesis and degradation of extracellular matrix necessary for tissue repair. Clinically, scar tissue formation and subsequent stricture after urethral injury often result in troublesome problems to urologists. We intended to know the involvement of TGF-β_1 in the formation of stricture and histological changes after urethral injury of rats. We injured urethrae of 24 adult male Sprauge-Dawley rats(200-250g) by urethrotome and Dormia basket, and then observed histological changes and analysed TGF-β_1 mRNA levels by Northern blot. Northern blot analysis showed that TGF-β_1 mRNA was expressed for 1,3,and 5 days after injury. Fibroblasts and deposition of extracellular matrix were markedly increased on day 5. Reepithelialization was completed and urethral lumen was narrowed on day 10. Our results indicate that TGF-β_1 is involved in the formation of stricture after urethral injury and plays a critical role in early phase of tissue repair and fibrosis.
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